Abstract: Somatic Cell Counts (SCC) in milk of Holstein-Friesian (HF) cows were monthly determined by direct microscopic SCC technique for two years period. In total, 1,682 SCC data from 95 HF cows were analyzed by using repeated measures. Herd, lactation month, parity, milking time and daily milk yield effects on SCC were found to be statistically significant (p<0.01). The averages of SCC in milk for herds were between 319, 448 and 497,279 cells mL-1. SCC in milk increased as parity increased. The average SCC in milk from the evening milking had about 28,768 cells mL-1 higher than that of morning milking. Improving barn conditions, managerial practices, milking management, giving an extra care at the first month of lactation and also milking at uniform interval will help to decrease SCC in milk.