Abstract: The aimed of this study is to extract the essential oil from Strobilanthes crispus (Acanthaceae) and Lawsonia inermis Linn (Lythraceae) and to investigate the chemical constituents, antioxidant activity and cytotoxicity effects of these oils on several cancer cell lines and normal cell lines. From GC-MS analysis revealed that the presence of at least 28 components for Strobilanthes crispus and 23 components in Lawsonia inermis. The total antioxidant activity from (FTC) and (TBA) methods showed that the essential oils of both plants have higher antioxidant activity compared to α-tocopherol. The essential oil from Lawsonia inermis displayed the strongest cytotoxic effect on liver cancer cell lines (HepG2) with IC50 (concentration that inhibit 50% of cell proliferation) value of 24 μg mL-1. However, the essential oil from Strobilanthes crispus did not give any cytotoxic value against all the cell lines tested. No cytotoxic effects of both oils in normal cell lines. The essential oils from both plants can be used as nutraceutical supplement to increase antioxidant needed in body to enhance defence systems, especially towards the incidence of degenerative diseases. The essential oil from Lawsonia inermis can be used as supplement in cancer patients especially in liver cancer patients.