Abstract: This study was aimed to asses the promotive effect of maltose, sucrose, proline, charcoal etc. on frequency of regeneration and embryogenesis. Factors affecting somatic embryogenesis and subsequent plant regeneration in vitro in basmati rice cultures were highly genotype specific. The number, mass and morphology of the callus formed on the scutellum were dependent on the medium used. They were affected by the auxins, sugars, amino acids, growth regulators etc. Supplementation of the control medium independently with maltose (30 and 60 g L 1), proline (560 m g L 1), Elevated sucrose (60 g L 1), activated charcoal (2 g L 1), cefotaxime (100 mg L 1) enhanced somatic embryogenesis considerably. The nodular embryogenic calli were further confirmed through histological analysis and their plant regeneration ability. The plant regeneration was also influenced both by the genotype as well as the composition of the medium used for induction of plant regeneration. Higher plant regeneration was achieved on medium containing maltose (30 and 60 g L 1), proline (560 mg L 1), elevated sucrose (60 g L 1) and activated charcoal (2 g L 1). Plants obtained were phenotypically normal and identical to control seed derived plants and exhibited normal fertility. A limited humidity and an optimal aeration of the culture tubes enhanced the frequency of embryogenesis and plant regeneration.