Abstract: Twelve primers were used to characterize the genetic similarity among ten chickens which named RIR I, RIR II, Barred I, Barred II, Colombian Rock, Line-54, Blue Line, Maroon Line, Black Line and Brown Line provided by Ankara Poultry Research Institute-Turkey. The objective of this study were to determine PCR-based genetic similarity between lines. Totally, 35 bands were scored for the dendogram prepared on the bases of a similarity matrix using the UPGMA algorithma. The highest and lowest genetic similarities were found to be 0.3773 between Bar I and Maroon and 0.0899 between Col and Bar II, respectively.