Abstract: Two sugarcane (Saccharum Officinarum L) varieties namely, Katha and BL4 were used in this study in order to compare their response for culture establishment, shoot proliferation, root induction and growth retardation. Shoot number, mass and length were the growth parameters measured. Genotype dependent response was found against different growth conditions such as growth media composition and growth regulators. Liquid Murashige-Skoog (MS) media was found to be more suitable for culture proliferation as compared to the solid media of the same composition, suggesting a positive and favorable effect of aeration and homogenization on culture performance. A better organogenesis response i.e. maximum shoot length, shoot mass and shoot number was observed when explants were cultured on the media containing 4.4 μM BAP for both the varieties but BL4 appeared to be more responsive genotype to shoot proliferation as compared to katha at any given level of growth regulator. Growth retardation was best achieved on the media containing 4 g L -1 mannitol among the concentrations used and degree of growth retardation was also found to be genotype dependent. There was a linear relationship between degree of growth retardation and concentration of the osmotica used. Root induction response (root number and root length) was the highest in katha cultures grown on half strength MS media containing 1.9 μM Indole butyric acid among the auxins, Indol butyric acid, indole acetic acid and naphthalene acetic acid, that were used in this study.