Abstract: Sudanese cattle and sheep were compared as to plasma, liver and urinary ascorbic acid concentrations. Cattle had lower hepatic ascorbic acid concentrations than sheep. Male cattle had lower plasma ascorbic acid levels and female cattle had lower urinary levels than their sheep counterparts. Concentrations of liver ascorbic acid were lower in females and urinary ascorbic acid was higher in females, irrespective of the species. It is concluded that cattle and sheep may differ as to ascorbic acid metabolism and status, but differences in environmental factors could have had an impact also.