Abstract: An investigation was carried out on the fish species availability in the fish landing centers of Khulna district. Almost all major fish landing centers in this area were surveyed. A total of 139 inland and marine water fish and crustacean species were observed. Out of 139, 126 species belonged to fin fish and the rest 13 species to crustacean. Amongst the fin fish species, 53 were fresh water, 23 were brackish water, 11 exotic and 39 marine water species. Twenty two fish species were found both as inland and marine water species. Nineteen species were detected endangered (Ompok pabda, Hilsa toil, Puntius sarana, Notopterus chitala, Mustus aor, Rita rita, Nandus nandus, Eutroeiichthys vacha, Notopterus notopterus, Wallago attu, Channa marulius, Labeo bata, Pangus pangus, Bagarius bagarius, Rasbora rasbora, Puntius ticto, Rohtee cotio, Labeo calbasu and Chanda nama). In summer, 30 species and in winter, 43 species were more available and the rest of the species were found all the year round. On the basis of abundance, Carp species, Lates calcarifer, Pelamys chiliensis, Trichiurus haumela, Katengus typus, Penaeus monodon and Scylla serrata were recorded most abundant, respectively.