Abstract: Some Bangladeshi indica rice (Oryza sativa L. var.) cultivars were subjected to tissue culture to study their regenerability in terms of regeneration percent and total number of regenerated plantlets obtained for a fixed sample size per variety. Regeneration potential was found to be the highest (88%) for BR-8 and the lowest (22%) for BR-31. Highest number of plants per seed were obtained for BR-8 and lowest for BR-31. Highly regenerating Indica rice cultivar BR-8 was subjected to genetic transformation mediated by Agrobacterium tumefaciens EHA105 harbouring the virulent plasmid pCAMBIA1301. Successful transformation events in the infected calli with this strain were followed by transient GUS assay using 5-bromo-4-chloro-3-indolyl β-D-glucuronide (X-gluc) as a substrate. The frequency of transformation in terms of transient GUS assay was found to be 40%.