Abstract: Leaf segments from field grown mature plants of Elaeocarpus robustus Roxb. were used to initiate cultures for inducing callus and subsequent differentiation of shoot buds. Callus production was obtained within seven weeks and adventitious shoot formation was observed after five weeks of incubation on ½ MS medium supplemented with 0.5 mg l-1 BA + 0.1 mg l-1 NAA + 15% CW. Complete plantlets were obtained upon transfer of shoot cuttings on ½ MS with 0.1-2.0 mg l-1 IBA, NAA, IAA or without any auxin. The best and healthy rooting was observed on ½ MS medium supplemented 0.2 mg l-1 IBA. In vitro regenerated plantlets were transferred to potting soil and successfully established under natural condition with about 60% survival.