Abstract: One year Pot culture experiment on Soybean in Soil + FYM (3:1) was conducted in medium textured and salt free soil of Quetta, Balochistan. Seven different treatments (T) of fertilizer were applied to both non-inoculated (non-inoc) and inoculated (inoc) set of experiment. T1 was kept control; T2 contained 23 + 60 + 30 kg NPK ha-1 and from T3 to T7 N fertilizer in the form of urea was added @ 25, 50, 75, 100 and 125 kg ha-1 along with combination of the same constant dose of P2O5 and K2O respectively. After physiological maturity, seeds were analyzed for their mineral N contents. Results showed that fertilizer treatments in general significantly, but negatively influenced the NO3-N and NH4-N, but reverse was true for NO2-N. A minimum level of NO3-N was recorded in T4 (0.065 μ M), NH4-N in T7 (0.133 g kg 1) and a maximum level of NO2-N in T5 (1.864 g kg-1). While by comparing the inoc with non-inoc treatments in particular doses of fertilizer, inoculation in general significantly and positively influenced the total mineral N content of mature soybean seeds. A maximum level of NO3-N was noted in T4 (0.122 μ M), NH4-N in T1 (0.466 g kg-1) and NO2-N in T4 (4.314 g kg‑1). However, by comparing the marginal mean values, inoc seeds comparatively produced greater amount of total mineral N content than that of non-inoc seeds. It is therefore safely concluded that low level of NO3-N followed by NH4-N in non-inoc seeds might have preferentially incorporated it into various nitrogenous organic compounds during the entire course of seed development to physiological maturity. The correlation coefficient (r) studies revealed that NO3-N and NO2-N were significantly and positively correlated with oil (0.445 and 0.489), but non-significantly correlated with protein (-0.020 and 0.041) and grain yield (0.210 and -0.206) respectively. While reverse was true in case of NH4-N association.