Abstract: The present investigation was designed to assess the role of temperature, an important environmental parameter on the chemotaxis Vibrio cholerae 0139 towards homogenates of Anabaena sp., amino acids (L-serine and L-alanine) and a carbohydrate (D-glucose). A series of temperature (4, 25, 37 and 44 ° C) was assessed in a capillary tube technique to observe its effect on the chemotaxis of V. cholerae 0139 at six different incubation periods of 15, 30, 45, 60, 75 and 90 min. The highest effect was observed at 25 ° C irrespective of the agents tested followed by 37 and 44 ° C. However, no significant chemotactic movement was recorded at 4 ° C. Among the various types of attractants used, maximum response was observed with L-serine as compared to L-alanine and D-glucose and Anabaena sp. homogenates.