Abstract: Excised-leaf water loss was studied in 50 F5 bulk lines in five wheat crosses including their common parent. A wide range of variability for excised-leaf water loss recorded as 5.47 to 18.81% and 10.36 to 25.36% at 2 and 4 h after excision, respectively. Generally, the lines that higher water at 2 h after excision also losses higher at 4 h after excision. For excised-leaf water loss the heritability was very high ranging from 89.36 to 96.70% in the five crosses. The result suggested the involvement of additive genes in the control of excised-leaf water loss. In general a negative correlation was found between excised-leaf water loss and yield in all the crosses. Among the five crosses Kanchan x DSN-34 provided maximum high yielding lines with low excised-leaf water loss.