Abstract: The experiment was performed in the experimental land of the Institute of Biological Sciences, Rajshahi during the period of April-May, 2000 and 2001. The physicochemical compositions of jute leaves under different foliar treatments were investigated at the mature stage. The results obtained showed significantly increase in most of the chemical composition under different treatments. The soil of the cultivated land as well as jute leaves obtained from experimental land became less acidic as compared to those of control field after application of NPK-fertilizers and cowdung in combination with foliar spray. The highest percentage of increase of jute plant and physico-chemical composition of leaves as compared to those of control were as follows: length of jute plant 38.35%, area of jute leaf 160.00%, specific gravity 6.48%, dry matter 27.78%, ash content 18.55%, total chlorophyll 42.17%, total sugar 69.47%, reducing sugar 48.15%, non-reducing sugar 72.90%. Only the moisture content of jute leaves from control jute plant was about to higher as compared to that from experimental jute leaves.