Abstract: A solution culture experiment was carried out to study the effect of calcium on the concentration of Na+, K+, Ca+2 and Cl‾1 ions in barley under saline conditions. Barley varieties Jao-83, Jao-85 and AS-54 were germinated. Three levels of salinity; control (0-40 me l‾1 = S1), 200 me l‾1 (S2) and 400 me l‾1 (S3) were created by using NaCl. In addition, CaCl2 was also added at the rate of 0, 10, 20 and 40 me l‾1 to all the salinity levels replacing equivalent amounts of NaCl. Seedlings were uprooted after 45 days of salination; root and shoots were cut separately and analyzed. Increase in each level of external salinity caused an increase in sodium and chloride concentration in barley shoot and root, while the concentration of potassium and calcium decreased with progressive increase in the substrate salinity. Addition of calcium at all salinity levels decreased sodium concentration but increased chloride, potassium and calcium concentration both in root and shoot. The variety Jao-83 contained lesser concentration of sodium and chloride and more of calcium and potassium as compared to Jao-85 and AS-54.