Abstract: The present study is concerned with the time course profile of citric acid fermentation by Aspergillus niger and its kinetic reletions. Maximum amount of anhydrous citric acid (70.60 g l-1) obtained, 144 h after inoculation, with a sugar consumption of 88.40 g l-1. The dry weight of mycelia was 17.39 g l-1. On the basis of comparison of kinetic parameters namely the product and growth yield coefficients (Yp/s, Yp/x), volumetric rates (Qq) and specific rate constants (qp), it was observed that mutant strain of Aspergillus niger GCB47 was a faster growing organism and has the ability to hyper produce citric acid.