Abstract: Lactuca is an important genus of tribe Lactuceae in Asteraceae. In Lactuca serriola the chromosome number, various meiotic stages, formation of pollen grains and pollen morphology is taken into account. Meiotic studies indicated that basic number of chromosomes is nine, n=9, (2n=18). In this species the meiosis was regular and pairing of homologous chromosomes was normal. During anaphase all the chromosomes moved regularly to the opposite poles. The chromosome bivalents are pronounced in diakinesis. Tetrad developed into pollen grains, whose equatorial and polar views were observed. The pollen grain is trizonocolporate in polar view. The pollen grain is echinate or spinate. It is recommended that the techniques like karyological and palynological study in connection with morphology must be carried out in correlation with the taxonomy on larger scale to find the variation and evolution in native species of the genus.