Abstract: The effect of three different auxins viz, naphthalene acetic acid (NAA), indole acetic acid (IAA) and indole butyric acid (IBA) each at four levels (0, 0.1, 0.5 and 1.0 mg l-1) was evaluated on meristem culture of Potato (Solanum tuberosum L.) for production of virus-free plantlets. Maximum plantlet height (8.3 cm), largest number of nodes/plantlet (7.3) and highest number of leaves/plantlet (8.9) were recorded at 0.5 mg l-1 of NAA followed by IBA at 1 mg l-1, whereas extensive number of roots/plantlet (23.7) as well as the earliest microtuber formation (17 days, after transplantation) were recorded at 1 mg l-1 of IBA followed by NAA at 0.1 mg l-1. Largest root (4.2 cm) was observed for IAA at 1 mg l-1 concentration. Enzyme Linked Immunosorbent Assay (ELISA) test proved that all plants were free of Potato Virus X (PVX), Potato Virus Y (PVY) and Potato Leaf Role Virus (PLRV), except one which was positive for Potato Virus X (PVX) virus.