Abstract: Gamma-rays induced mutation analysis was conducted on Drosophila simulans grown on the standard maize meal medium. The un-etherized young males (2-3 days old) were exposed to 2000 r gamma radiation and were crossed to controlled virgin female flies, on the same day. The F1, F2 and F3 generations were examined to identify visible mutations. A total of five induced mutant flies were identified and isolated from the irradiated strains. Three out of the five induced mutants were cultured successfully and their genetic pattern was studied, while the remaining two induced mutants could not be cultured due to unavoidable circumstances. The cultured mutants comprised one sex-linked recessive, and two autosomals (one autosomal dominant and one autosomal recessive). No spontaneous mutant fly could be noticed in the non-irradiated culture grown for comparison.