Abstract: A series of experiments to explore genotypic behaviour of the two F1 tomato hybrids against a multitude of hormonal regimes were conducted. The diverse explant sources like shoot apices, nodal segments and root sections were subjected to MS media containing different kinds and concentrations of plant growth regulators. Callus induction from cut surface of the shoot tips was more on BAP-IAA enriched media than Kin-IAA enriched media in both hybrids. Both hybrids regenerated 3-7 shoots per culture at Kin-IAA enriched MS media whereas it was 2-3 shoots per culture at BAP-IAA enriched media. A minor callus induction from nodal explants was observed in Kin-IAA enriched media in hybrid Bornia whereas Royesta failed to induce callus. Bornia regenerated 1-2 shoots/culture on Kin-IAA enriched media where as Royesta induced 1 shoot/culture on both media tested. Both hybrids produced non-embryogenic calli from root segments at 100% frequency on MS containing IAA at all levels tested whereas they exhibited a low callus induction frequency on 2,4-D enriched media. Clusters of long roots were found on IAA enriched MS media while no such response was observed on 2,4-D enriched MS media.