Abstract: Background and Objective: Isomerisation is one of the main processes in the refinery to convert light naphtha such as n-hexane into their respective isoparaffins with high octane number. In this paper the performance of locally prepared light-naphtha isomerization catalyst MoO3/TiO2 was evaluated in a pilot-scale and compared to Platinum on Zeolite (Pt/Z) commercial catalyst under different operating conditions such as temperature, pressure, hydrogen to oil ratio and liquid speed velocity, using normal hexane (nC6). Materials and Methods: BothMo2O3/TiO2 and Pt/Z catalysts were tested in the pilot plant designed by the Petroleum Research Center (PRC), The performance of the catalyst was tested under the selected conditions then compared to the performance of a commercial Pt/Zin the same pilot plant under the same conditions. Results: The MoO3/TiO2 showed acceptable performance for converting the nC6 to six carbon atom paraffin isomers (SCA P-isomer) the 2-methyl pentane and the 2,3-dimethylbutane (23DMB). The MoO3/TiO2 operates steadily within the pressure range 5 to 10 bar, but at higher pressures, its cracking function dominates to unacceptable levels. Pt/Z, unlike the MoO3/TiO2, did not show a tendency to form the formation of the benzene and heptane and its cracking function reached 3.1% only at 290°C, which is the catalyst maximum operating temperature. Conclusion: The stability test showed that the Pt/Z is stable with time on stream and change in reaction temperature, whereas the MoO3/TiO2 performance was considered sensitive to temperature change.