Abstract: Background and Objectives: Accumulation of heavy metals in soil and plant tissue is one of the most pressing threats to the growth and development of plants, as well as human health. The purpose of the investigation was to evaluate the impact of using treated and untreated industrial effluent on the amount and type of heavy metals accumulated in soil and plant tissue and its impact on growth and yield of sweet potato (Ipomoea batatas) va. Kulfo. Material and Methods: The experiment was conducted at Hawassa College of agriculture using pot at field condition during January-June, of 2016. A completely randomized experimental design with 4 treatments (3 replicates each) were used. The effluent samples were collected from; untreated lagoon, semi treated lagoon, treated lagoon and Lake Hawassa water and used as treatments. Plant growth and physiological parameters were collected during the vegetative growth period and during harvesting time (150 DAP). Results: The result indicated that sweet potato irrigated with untreated textile wastewater significantly reduced vine length as compared to control. Plant irrigated with semi treated wastewater gave maximum photosynthesis and transpiration rate than untreated, treated and water from Lake Hawassa (Control). The bioaccumulation concentration factor (BCF) value of Cu in untreated wastewater was 1.4-2 fold higher than the translocation value Cu in lake water, semi treated and treated treatments. Copper (Cu) has higher capacity to accumulate in edible parts of sweet potato tuber than Cd, Cr and Zn metals. However, Zn had stronger potential to accumulate in soil than Cu and Cr. Conclusion: It is concluded that both Zn and Cu were found potential metals to accumulate both in soil and plant tissue and likely threat for human health than Cr if inhabitants of study area experience the consumption of sweet potato irrigated with untreated textile effluent.