Abstract: Background and Objective: Parachanna obscura is a member of African Channidae with great aquaculture potential in west Africa. The knowledge on chemical composition of P. obscura as food is very poor. Therefore, this study was carried out to determine the proximate composition and mineral contents of P. obscura from Eleyele reservoir, Nigeria. Materials and Methods: Three live samples of each sex (382.42±59.41 g) were collected monthly for one year. Samples were segregated according to sex and season and analyzed for proximate composition and mineral contents using AOAC and Atomic Absorption spectrophotometry methods. Data were analyzed using descriptive statistics and one-way ANOVA at α0.05. Results: The biochemical profile of P. obscura revealed high crude protein (23.19±1.06%), low fat (1.09±0.06%) and good level of carbohydrate (5.13±0.20%) and ether extract (7.00±0.26%). The results showed significant seasonal variation (p>0.05) in biochemical compositions but no marked variation (p<0.05) between sexes for all the samples examined. The highest proximate composition and mineral contents were recorded in wet season samples except for plasma ions (Na and K). Conclusion: The study showed that P. obscura has good nutritional contents with better nutritional value in wet season samples.