Abstract: Background and Objective: There has been not much information available on phytochemical components and biological activity in the bark and leaves extract of Barringtonia acutangula L. The objective of this study was to evaluate in vitro antioxidant activity, antimicrobial activity and characterize the chemical constituents of leaves and bark extract of Barringtonia acutangula L. by using gas chromatography-mass spectrometry (GC-MS). Materials and Methods: This investigation was carried out to determine the possible chemical components from Barringtonia acutangula L. by GC-MS.-QP 2010 PLUS SHIMADZU JAPAN. The antioxidant activity of plant material was assayed by reducing power assay. Results: This analysis revealed that the ethanol extract of Barringtonia acutangula L. (Bark) contained 32 phyto components, mainly 1,2-Benzenedicarboxylic acid diethyl ester, Dibutyl phthalate, 1,2- Benzenedicarboxylic acid, etc. The leaves extract contained 37 phyto components, among them 2,6,10-Trimethyl, 14-ethylene-14-pentadecene, 4,4a,6b,8a,11,11,12b,14a-Octamethyl-eicosahydro-picen-3-one, Dibutyl phthalate, 1,2- Benzenedicarboxylic acid, Friedelan-3-one were the major components. The reducing power of the plant extract was compared with the standard ascorbic acid and found to be dose-dependent. Conclusion: From the results, it is evident that Barringtonia acutangula L. contains various bioactive compounds and is recommended as a plant of phytopharmaceutical importance.