Abstract: Background and Objective: The housefly (Musca domestica L.) is a cosmopolitan dipterous fly and considered as one of the most important insects that transmits pathogens of a considerable medical and veterinary importance. The control of parasitic stages of such fly is a major potential target. This study was conducted to evaluate, in vitro, the efficacy of five compounds against the third larval stages of M. domestica; two natural products (peppermint oil and Moringa oleifera extract), two commercially used insecticides (Butox® and phoxim) and a commonly used disinfectant, Virkon®S. Materials and Methods: The third larval stages of M. domestica were collected and underwent the larval bioassay, Bait method (food-media technique). Both lethal concentrations (LC50 and LC90) of tested compounds were detected 24 h post-treatment. Moreover, the potential effects on larval mortality, larval duration, pupal duration and adult emergence as well as detectable abnormalities were assessed. All data were statistically monitored. Results: It has been found that phoxim, peppermint oil and Butox® were more toxic with significant LC50 and LC90 values. Oppositely, both Virkon® S and Moringa oliefera extract 10% had no effect. Efficiently, the use of the peppermint oil induced a prolonged larval duration and a lower larval survival rate with prominent morphological abnormalities in all parasitic stages. Phoxim was more potential as larvicidal than Butox® with slight morphological abnormalities at the pupal stage. Conclusion: The present investigation revealed that compared to commonly used insecticides, the natural product, peppermint oil (Mentha piperita), is an effective, safe and cheap larvicidal against the third larval stages of houseflies and it could serve as an eco-friendly housefly control measure, implying that more studies dealing with other natural substances against dipterous flies of veterinary and medical importance are requested.