Abstract: Background and Objective: Anti-biotic resistance has been increasing in prevalence, thus becoming a major medical challenge worldwide and posing a big threat to human society. Hence, it becomes essential to search for newer drugs with lesser rate of resistance development and lesser toxicity especially from plant resources. Materials and Methods: Mitracarpus villosus plant was obtained within Aliero, Nigeria, while the multi-drug-resistant bacteria (Staphylococcus aureus, Klebsilla pneumoniae, Streptococcus pneumoniae and Salmonella typhi) used were obtained from Sir Yahaya Memorial Hospital Birnin Kebbi, Nigeria. Acetone extract of the plant was used and test organisms were taken from nutrient agar slants and sub-cultured on nutrient agar plates. The anti-bacterial activities were conducted using agar well diffusion method. Results: The plant phytochemical composition shows the presence of saponins, tannins, alkaloids, terpenoids, steroids, anthraquinones, flavonoids and phenols. The result for the anti-bacterial activity reveals that, the zones of inhibition at extract concentration of 120 mg mL–1 were 5.89±0.38 mm for S. aureus, 5.22±0.19 mm for S. pneumoniae, 6.89±0.70 mm for K. pneumoniae and 6.11±0.70 mm for Salmonella typhi. The higher the concentration of the plant extract the higher the activity and at lower concentration, the activity reduces significantly. Conclusion: The results obtained revealed that, M. villosus has anti-bacterial activity against the tested multi-drug-resistant micro-organisms, which was attributed to the presence of some of the phytochemicals in the plant extract.