Abstract: Background and Objectives: The exploration of fruit biomass derived biomaterials (bio-antiseptic, biosolvent, biofilm, biofuel) encourages the uses of biomass extensively. Thus, fruits wastes can be reused to generate bioethanol as antiseptic and bio-solvent. The study was carried out to investigate the optimization of bioethanol production and evaluate the bioethanol as anti-fermenter and anti-septic. Materials and Methods: Rotten banana, grape and dates biomasses were used through fermentation bioprocess using yeast. Samples were thoroughly washed with distilled water, cut using a sterile knife and were blended by using a sterilized automatic juice blender. Results: Bioethanol yield was higher in dates biomass than in grape and banana biomass at 3 g L–1 yeast concentration at 30°C. The lowest pH was found in the bioethanol produced from dates biomass. The lower TSS and glucose content was exhibited in the bioethanol produced from banana biomass. The lowest viscosity and acid value was found at 3 mg L–1 of yeast concentration in dates biomass. Chemical elements like Ca, P, Fe, Pb, Cu and Si fulfilled the requirement of the standard specification as well. Grape juice mixing with bioethanol showed antifermenter for 2 days while in the 1st day juice started to rot the faster in the control. The lowest bacterial colony formation was observed in the dates biomass derived bioethanol. Conclusions: Results explored that produced bioethanol was of good quality and can be used as antiseptic and bio-solvent from fruit biomass.