Abstract: Background and Objective: Gymnema sylvestre is a perennial climber, well known for its pharmaceutical value. Despite of several studies on chemical composition and biological activities, there is a meagre information available on agronomical trails on this species. To analyze the effect of organic, bio and NPK fertilizers on the plant growth and leaf yield. Materials and Methods: Four treatments viz. T1: Application of 100 kg of Farm Yard Manure; T2: Application of 1.2 kg nitrogen, 0.8 kg phosphorus and 0.4 kg potassium, T3: Application of 6.75 kg well decomposed coco peat, T4: Application of 0.10:0.10:0.08 kg of Azospirillum:Phosphate Solubilizing Bacteria (PSB):Vesicular Arbuscular Mycorrhizal (VAM) in each replicate having unit area of 100 m2. Data on plant height, number of branches per plants, dry weight of leaf yield per plant and per unit leaf yield was recorded in 12, 18 and 24 months after transplantation (MAT). Results: Maximum plant height of 188.96 cm was recorded in T2 for 12 MAT. For 18 and 24 MAT, maximum plant height of 293.29 and 326.65 cm, respectively was recorded in T4. Likewise, average number of branches per plant was recorded maximum of 7.85 in T2 for 12 MAT, 19.36 and 22.46 for T4, respectively in 18 and 24 MAT. Highest dry leaf yield of 124.84 kg per acre was achieved in T2 for 12 MAT, while for 18 and 24 MAT, highest yield of 348.84 and 381.44 kg was achieved in T4. Conclusion: Combine application of bio-fertilizers (Azospirillum, VAM and PSB), significantly improves the growth and yield in Gymnema sylvestre.