Abstract: Background and Objectives: Hepatitis C becomes a problem in liver disease over the world. The diagnosis of hepatitis C infection is carried out by detecting of antibodies against the virus using an immunoassay but unreliable in the seroconversion phase of infection, due to the long window period. Real time PCR examination and hepatitis C virus core antigen (HCVcAg) detection by ELISA offer an alternative method to detect the virus infection. The aim of this study was to identify the viral load correlation with the number of HCV core antigen and aminotransferase levels in HCV infection. Materials and Methods: This study combined the molecular examination for determining the genotype of HCV and viral load with q-PCR and ELISA method for investigating the HCVcAg. The correlation between number of viral load with HCVcAg and Alanine aminotransferase (ALT) and aspartate transaminase (AST) were analyzed with Pearson’s correlation test. Results: There was a significant correlation between the number of viral load with HCVcAg levels in hepatitis C patients but levels of ALT (49.71 U mL–1) and AST (31.83 U mL–1) serum were not correlated with the number of viral load. Conclusion: HCVcAg can be applied as an alternative examination to detected viral load in hepatitis C infection to prevent the infection of HCV lead to cirrhosis and even hepatocellular carcinoma.