Abstract: The purpose of this study is to investigate the influence of the regulatory adaptation on sport performance. Regulatory Focus Theory thinks that there are two different focus orientations in both individual and environment threat which are promotion focus and prevention focus, respectively. The past studies have verified that when individual’s focus orientation is coincident with environmental focus orientation (regulatory adaption), the optimum effect occurs, this conclusion is also verified by many other studies. However, in the sports context, very few researchers applied this theory to explore its effect on sport performance. In view of this, sixty college students were recruited as subjects and conducted simple throwing experiment to examine whether the optimum effect exists when regulatory focus of individuals and situation become adapted. This study measured individual’s regulatory focus at first and then manipulated the regulatory focus in the procedure of experiment design. The result of this study supports the presented hypothesis: The influence of relative strength of individual’s focus to throwing performance was adjusted by experiment manipulation. Put it plainly, the result shows that with the manipulation of promotion focus, the influence of high relative strength to sport performance is enhanced, similarly, with the manipulation of prevention focus; the influence of low relative strength to sport performance is enhanced too. Overall, the results of this study supports that regulatory focus adaption have an influence on the sport performance.