Abstract: In this study chitosan beads were synthesized and cross-linked using glutaraldehyde in order to enhance the chemical resistance and mechanical stability of chitosan beads. Herein the biopolymer chitosan-GLA beads were used as a support for Au nanoparticles. This process contains two steps; using chitosan-GLA beads to adsorb Au(III) ion from aqueous solution of HAuCl4 followed by reduction of adsorbed Au(III) ion to Au(0) using the aqueous Psidium guajava leaf extract as reducing and stabilizing agent. The chitosan-GLA/Au(0) characterized using Fourier Transform Infrared (FTIR) spectroscopy, field emission scanning electron microscope/energy dispersive scanning electron microscopic (FESEM), (EDX), X-Ray Diffraction (XRD) and BET surface area analyses which proves the successful immobilization and reduction of Au(III) ion to Au(0) form.