Abstract: The possibility of using Oil Palm Trunk (OPT) as new material for MDF production was investigated. Important factors that contribute to the quality of the MDF made from OPT are the parenchyma content, density and resin content. The parameters studied for the MDF prepared from OPT are the density (650 and 750 kg m-3), parenchyma content (10, 20 and 30%) and Urea Formaldehyde (UF) content (8, 9 and 10%). Thickness Swelling (TS), Internal Bonding (IB), Modulus Of Rupture (MOR) and Modulus Of Elasticity (MOE) of the test panels were determined according to European Standard EN 622-5:2006. Density and parenchyma content were found to have great effect on the properties of MDF panels meanwhile, the resin content have significant effect all panel properties except on the MOE. The results indicated that MDF panel produced at 750 kg m-3 is better than that of produced at 650 kg m-3. Panels with low parenchyma content (10%) showed better dimensional stability with good bonding and bending strength compared to the panels with higher parenchyma content. For resin content, an increase in UF content resulted in decrease of TS and increase of IB, MOR and MOE. In general, MDF prepared at 750 kg m-3 with 10% of parenchyma content and 8% of UF content exhibits the best properties, which exceed the level requirement in the existing standard for fibreboard.