Abstract: A henhouse carbon dioxide monitoring system was designed based on WSN to collect henhouse different location carbon dioxide. The system composed of hen atmega16L processor, nRF905 wireless communication module and S-100 CO2 sensor module was developed. The system had the function automati collection, storage, and wireless transmission henhouse different points carbon dioxide. The experiment showed that: The system power can work more than 3 months , and the system has achieved effective henhouse carbon dioxide acquisition on different location.