Abstract: As a kind of extreme phenomenon of negative stress, job burnout is eroding the physical and psychological health of the R and D employees in the high-tech enterprises. The empirical results show that the R and D employees in the high-tech enterprises remain some degree of job burnout. The level of the R and D employees’ job burnout is remarkably different in the demographic variables, such as the length of service and their position. Pay-reward imbalance, unfair appraisal, incompetence, lack of work autonomy and career development are the main work stressors experienced by the R and D Employees which have significant positive correlation with job burnout. To some extent, organizational support has affected the level of the R and D employees’ job burnout. So, the managers may effectively reduce the level of the R and D employees’ job burnout by establishing a fair appraisal system, improving the R and D employees’ reward, helping the R and D employees to grow professionally and providing support to the R and D employees.