Abstract: Presently Chinese agri-food cold chain logistics industry is just at the initial stage which scarce a reasonable method to evaluate regional competitiveness. On the basis of multivariate statistical data, an assessment indexes framework for evaluating is established which is composed of three hierarchical levels, four subsystems and twenty assessment indicators. The established system is applied to evaluate regional agri-food cold chain logistics competitiveness with exemplification of Heilongjiang province, People’s Republic of China, on which the competitiveness of the 13 prefecture-level cities is comprehensively analyzed with application of principal component analysis method, meanwhile the relevant classification is realized by system clustering. As a result, the related countermeasures for the respective layers to improve their competitiveness are put forward.