Abstract: Reconstituted collagen film formed from lyophilized collagen in acetic acid and deposited on glass slide substrate was found to be randomly orientated. In this study PVDF and its copolymer (PVDF-TrFE) were used as a template for depositing collagen, a type of protein abundantly found in human body. The objective of this study was to develop the nucleation and growth of the aligned PVDF and PVDF-TrFE crystals. Collagen films formed, showed strong affinity to these fluoropolymers. The PVDF/PTFE fibres initiated growth of collagen films present in coiled collagen twisted structures around the PVDF and PVDF-TrFE fibres. The thickness of the collagen on the PTFE film obtained from AFM approximately 15 nm. Upon deposition of collagen on PVDF-TrFE/PTFE films, the thickness increased to 40-110 nm. The collagen was observed to orientate in the direction of PVDF-TrFE/PTFE. The collagen film formed on 72-28 PVDF-TrFE/PTFE film template was observed to be relatively less disorder and can be used in biomedical application. The dielectric properties of the collagen may be investigated for the application in electro-mechanical applications.