Abstract: The objective of this study was to screen, identify and characterize of cellulolytic fungi from various soil management fields; organic, young organic, semi-chemical and chemical soil from Surin rice fields, Thailand. Fungi from various type of soils were isolated by dilution plating technique on Reese minimal medium supplemented with cellulose powder and rice straw then incubated at 25°C for 3-7 days. The isolated fungi were screened and identified using slide culture technique. The enzymatic activities were assessed by qualitative method for cellulase, xylanase, peroxidase and laccase activities. Two-hundred and fifty-eight fungi isolates found in Surin rice fields belonging to the genus Penicillium (5 species), Paecilomyces (4 species), Aspergillus (3 species), Acremonium (2 species), Chaetomium (2 species), Alternaria (1 species), Bipolaris (1 species), Curvularia (1 species), Fusarium (1 species), Humicola (1 species), Mucor (1 species), Nigrospora (1 species), Phoma (1 species), Pyrenochaeta (1 species), Pythium (1 species), Rhizopus (1 species), Sporotrichum (1 species) and Trichoderma (1 species). Out of 29 fungal species clearly showed different in enzymatic activities. Most tropical soil fungi had ability to produce cellulase, xylanase, laccase and peroxidase, respectively. The highest capacity was found only in cellulase. Aspergillus niger, Aspergillus sp., Chaetomium murorum and Trichoderma sp. showed the highest potential to produce cellulase. Eleven species of soil fungi showed high capacity in xylanase activity. For laccase and peroxidase activity, there were found in 2 species. The results also revealed that only ten showed highest carboxymethyl cellulase, xylanase, peroxidase and laccase activities by qualitative screening method for enzymatic assay. They were identified as Aspergillus niger, Acremonium sp., Aspergillus sp., Chaetomium murorum, Humicola grisea, Mucor sp., Paecilomyces victoriae, Penicillium janthinellum, Penicillium lanosum and Trichoderma sp. These tropical soil fungi will be beneficial to use for biodegradation and decomposition of agricultural residues, especially rice straw.