Abstract: Heavy metals are generally toxic to microorganisms, especially if they exist at high concentrations. Environmental pollution particularly in soil with heavy metals can stem from industrial activities or sewage discharges. In this study, twelve Streptomyces species isolated from Egyptian soil and identified by using physiological tests as Bergey’s manual was assessed quantitatively to the effects of zinc using plate diffusion method. Biosorption of zinc by biomass microorganisms is an innovative and alternative technology for removal of these pollutants due to their good performance, low cost and large available quantities. Experiments in liquid culture were used to determine concentration ranges of the metals at which the most tolerant species could grow showed that Streptomyces aureofacienes had maximum uptake of zinc (734.8 μg g-1 biomass).