Abstract: The interaction between wheel-rail produced defects on each other. Many studies of wear were done on interaction between the wheel-rail proposed using pin on disk tribometer. The present study proposes extend research using pin on disk with normal loads. The research aimed to determine the influence of applied load toward wear rate and wear mechanism on rail materials. Wear rate is determined using volumetric loss, and wear mechanisms on interaction between wheel-rail can be analyzed using Scanning Electron Microscopy (SEM). Energy Dispersive X-ray Spectroscopy (EDX) supposed to detect possibilities the presence of other elements on debris wear. The results show wear rate increase linearly from 2.27, 3.40 and 6.81 mm3 km-1 on 20, 40 and 60 N applied load, respectively. Experimental result shows that the wear rate increases 0.1135 in proportionally with the increasing of applied load. Wear mechanisms begin from abrasive wear of both contact surfaces, then following severe adhesive wear. EDX analysis shows presence of the third body from internal and external elements of contact surface material.