Abstract: Attempt was made in this research to extract the oil from Monopterus albus or commonly known as eel by using assisted ultrasonic extraction method. The effects of different ultrasonic power, solvents volume and sonication time on extraction yields were investigated. The ultrasonic power used was 100, 200, 300 and 450 Watts. The amount of ethanol or solvent used was 50, 100, 200 and 500 mL. In terms of sonication time it was varied at 20, 30, 50, and 60 min. Analysis was done using 785 DMP Titrino and Gas Chromatography Mass Spectrometer (GC-MS). Results obtained show that the maximum ultrasonic power of 200 Watts, ethanol volume of 500 mL and 60 min of sonication time produce the higher yields of oil extracts. The yields were 7.20% with FFA content of 0.22 (g/100 g). The FFA detected from chromatographic analysis using GC-MS was palmitic acid and stearic acid.