Abstract: In today’s world the energy crisis is the main concern. It has been tried to develop substitute materials of petroleum and other decreasing natural fuel resources. Biomass is an abundant and renewable resource and it impelled researcher to develop suitable and proficient ways for converting it to valuable chemicals, biofuels and biomaterials. Due to their several advantageous properties ionic liquid was used for the dissolution of biomass. Biomass after dissolution in ionic liquids was regenerated with reconstitutes solvents (acetone/water). The regenerated material was characterized by FT-IR, XRD and SEM and compared with untreated biomass. The regenerated material was found to exhibit more homogeneity in the microstructure and the fibrous nature of material disappeared after dissolution. The crystallinity of cellulose was changed from cellulose I to amorphous during dissolution and regeneration from ionic liquids.