Abstract: This study reported the influence of shear rates on the performance and morphology of asymmetric UF membranes for Bovine Serum Albumin (BSA) separation. Flat sheet UF membranes were fabricated from a dope solution with 17% polymer concentration and manipulation of shear rates was done at 176.2, 234.98, 352.47 and 704.9 sec-1. Fabricated UF membranes have been characterised in term of pure water permeability, membrane morphology and membrane pore radius. The membrane performance was determined based on sodium chloride and BSA rejection profile. This study has proposed that a fabricated membrane with 704.9 sec-1 shear rate is the most appropriate membrane for BSA separation when it achieved the highest flux around 59.7 L m-2 h and BSA rejection of about 100% at an optimum pressure of 10 bars. Observation proved that higher shear rates applied during the membrane casting process would promote a significant effect on the performance and morphology of UF membranes and lead to an upgrade in membrane selectivity.