Abstract: This study aimed to investigate the hygienic conditions of air at delivery and nursing rooms at some hospitals located in Khartoum district Sudan. Out of seventy-nine randomly collected air samples from delivery rooms at the four largest hospitals (Khartoum Teaching Hospital, Al Ribat Hospital, Ahmed Fadul Hospital and Omdurman Maternity Hospital), 63.3% showed positive bacterial growth. Out of 60 air samples that were collected from the nursing rooms, 66.7% of them showed positive bacterial isolation. The isolated bacterial species were identified as S. aureus, E. coli, Klebsiella sp., P. aeruginosa and Bacillus sp. The highest percentage of bacterial isolation was recorded in Khartoum Hospital as 50.6 and 42% in delivery and nursing rooms, respectively. Staphylococcus aureus and P. eruginosa were the most dominant organisms isolated from the delivery rooms at all examined hospitals while S. aureus. showed the highest percentage from nursing rooms at two of the examined hospitals, E. coli and Bacillus sp. were dominant in Khartoum Hospital. The isolated bacteria showed clear resistance toward the common antiseptics used in hospitals.