Abstract: This study was aimed to evaluate the advantages of immobilization of Pycnoporus sanguineus on Ecomat for mycelial biomass yield, laccase production and hence, polycyclic aromatic hydrocarbons (PAHs) degradation. The addition of polycyclic aromatic hydrocarbons standard mixture increased the mycelial biomass yield of both immobilized and free mycelia of P. sanguineus with the highest dry weight obtained at 10 days of incubation of 0.46 and 1.88 g, respectively. The immobilized mycelia culture degraded 88% of phenanthrene, 93% of anthracene and 85% of pyrene within 20 days. In comparison, free mycelia culture rapidly degraded 42% of phenanthrene, 92% of anthracene and 87% of pyrene at the end of 20 days incubation period. The high correlation between the amounts of polycyclic aromatic hydrocarbons degraded and laccase activity of both immobilized and free mycelia culture indicated possible involvement of laccase in polycyclic aromatic hydrocarbons degradation.