Abstract: The resin oil obtained by hydrodistillation from Dacryodes edulis G. Don growing in Gabon was analyzed by GC and GC/MS. The major constituents in the essential oil were sabinene (21.76%), terpinene-4-ol (19.79%), α-pinene (17.47%) and p-cymene (11.29%). The in vitro antioxidant activity was investigated with two methods: 2, 2-diphenylpicrylhydrazyl radical (DPPH) scavenging essay and β-carotene bleaching test. Butylated hydroxyltoluene was employed as positive control. The essential oil showed antioxidant and DPPH radical scavenging activities and it displayed the inhibition of lipid peroxidation. Furthermore, the antimicrobial activity of essential oil was evaluated using a broth microdilution method. Dacryodes edulis essential oil exhibited antibacterial activity but it was unable to inhibit the growth of fungal species tested.