Abstract: In this work, palm based dihydroxystearic acid (DHSA) was produced from epoxidation of oleic acid with performic acid, followed by hydrolysis of the epoxide. The crude DHSA was purified by employing solvent crystallization. Two different crystallization conditions (natural cooling and rapid cooling modes) were studied using ethanol as solvent. Scanning electron microscopy (SEM), gas chromatography (GC) and particle size distribution (PSD) were used to investigate the physical and chemical characteristics of the crystals. The SEM revealed that DHSA forms a sphere-like crystal. After crystallization, purity of the DHSA increased from 69.3 to 86.8-91.4%. It was observed that natural cooling mode yielded a broader distribution and larger particle diameter compared to rapid cooling mode.