Abstract: The aim of the present study was to investigate the antimicrobial and cytotoxic activities of five newly synthesized ferrocene based complexes [Mn(FcCOO)2, A], [Co(FcCOO)2, B], [Ni(FcCOO)2, C], [Cu(FcCOO)2, D] and [Zn(FcCOO)2, E]. The maximum antibacterial (at the concentration 100 μg disc-1) and antifungal (at the concentration 200 μg disc-1) activities were shown by the manganese complex A followed by cobalt complex B. The minimum activites were shown by Zink complex E. The minimum inhibitory concentration of the complexes was determined against four pathogenic bacteria Bacillus subtilis, Bacillus megaterinum, Escherichia coli and Shigella shiga and the values of complex A were found between 16-32 μg mL-1. Brine shirmp lethality bioassay was carried out and all the complexes also showed cytotoxic effect compared with the standard bleomycin (0.41 μg mL-1).