Abstract: In this study, performances of three different electronic pulsator control unit were determined to over come the problems faced especially by pneumatic pulsators. For this reason, three different pulsation control units, one with conventional components (PCU1), one with PIC (PCU2) and one with PLC (PCU3) were used. All of the three units were designed to control a selenoid valve system and connected to a mobile milking machine performed milking for measurement of pulsation characteristics. A pulsator test equipment was used to determine the pulsation characteristics. Tests were carried out at 40, 5 and 50 kPa vacuum levels and 50, 55 and 60 min-1 pulsation rates. Variance analysis and Duncan tests were performed on experimental data, which can be effective on changes. Based on the result of statistical analysis, changes of different levels and interactions between pulsation rate and vacuum level were determined. It was determined that, most of these interactions were caused by structural properties of control units and selenoid valve. However, it was seen that pulsation characteristics obtained by each of three pulsation control unit were accordance with international standards at all vacuum levels and pulsation rates. In additional, results of detail statistical analysis showed that, PCU2 had more suitable characteristics than PCU1 and PCU3.