Abstract: In this study, High Performance Liquid Chromatographic method was used for the determination of ciprofloxacin, enrofloxacin, levofloxacin, norfloxacin, ofloxacin, flumequine, oxolinic acid and nalidixic acid. These compounds were extracted with meta phosphoric acid : acetonitrile (3:7), followed by a bond elute resins and n-hexane was used to remove fates. The detection limits of quinolones were between 1 to 4 μg on U.V detector. Good linearity was observed from the calibration the graph at concentrations from 2 to 400 μg. A comparative was made between the winter and summer seasons. It was found that the amount of residual quinolones in liver and kidneys were more than those in muscles and eggs.