Abstract: In this study, we present a novel approach to match a line segment in the sequence of images obtained by camera in motion. The proposed method is based only on the formalism of the slope of the segment in the sequence of images. We use the definition of the projection of points and the relation which exists between the slopes of the segment in various images. The approach developed not needs the calibration of camera. To resolve the matching problem, two segments in three images are necessary. Experiment showed feasibility as well as robustness of the algorithm.