Abstract: The aim of this study was to measure concentrations of bacteria and physical measurements and identify bacterial bioaerosols in poultry feed plant. In this cross-sectional study3 area samples were collected. The production line had the highest mean concentrations bacteria, temperature and relative humidity. The mean±SD concentrations of production line and office building collected were 113±4.1 and 40±1.4 cfu m-3 for bacteria, respectively. There were found significant differences between mean concentrations of bacteria and physical measurements at production line in comparison with the other locations and office building (p<0.0001). The genera were identified include Staphylococcus epidermis, Streptococcus pneumonia, Escherichia coli and Pseudomonas aeruginosa. We conclude that differences in bacteria concentrations among different locations within plant were generally significant. More research is needed to establish better exposure assessment tools and to validate newly developed methods.